Digital tissue diagnostics utilizes the power of image analysis to extract quantitative information from conventional pathology slides. However, it is a complex process consisting of many steps that can potentially lead to erroneous results. Before the sample is subjected to digital analysis the tissue must be collected and preserved, embedded in paraffin, sectioned onto glass slides, subjected to antigen retrieval, stained using chemical, immunohistochemical (IHC) or immunofluorescent (IF) techniques and then digitized with a slide scanner. , which on top of tumor heterogeneity and biological variability, .
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